Phospholipase C
Phospholipase C is a class of enzymes that cleave phospholipids just before the phosphate group (see figure). It is most commonly taken to be synonymous with the human forms of this enzyme, which plays an important role in eukaryotic cell physiology, particularly signal transduction pathways. Thirteen kinds of mammalian phospholipase C are classified into six models (β, γ, δ, ε, ζ, η) according to structure.
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Human variant
The human variant has EC 3.1.4.11.
Activation
Receptors that activate this pathway are mainly G-protein coupled receptors coupled to the Gαq subunit, including:
- 5-HT2 serotonergic receptors[1]
- α1 (Alpha-1) adrenergic receptors[2]
- Calcitonin receptor[1]
- H1 histamine receptor[1]
- Metabotropic glutamate receptor, Group I[1]
- M1, M3, and M5 muscarinic receptors[1]
Other, minor, activators than Gαq are:
- MAP kinase. Activators of this pathway include PDGF and FGF.[2]
- βγ-complex of heterotrimeric G-proteins, as in a minor pathway of growth hormone release by growth hormone releasing hormone.[3]
Effects
PLC cleaves a phospholipid. In the process, phosphatidylinositol 4,5-bisphosphate (PIP2) is cleaved into diacyl glycerol (DAG) and inositol 1,4,5-triphosphate (IP3). DAG remains bound to the membrane, and IP3 is released as a soluble structure into the cytosol. IP3 then diffuses through the cytosol to bind to IP3 receptors, particular calcium channels in the endoplasmic reticulum (ER). This causes the cytosolic concentration of calcium to increase, causing a cascade of intracellular changes and activity.[4] In addition, calcium and DAG together works to activate protein kinase C, which goes on to phosphorylate other molecules, leading to altered cellular activity.[4] End effects include taste, tumor promotion, etc.[4]
In other organisms
Other phospholipase C enzymes have been identified in bacteria and in trypanosomes, each with their own EC number.
- Phosphoinositide phospholipase C EC 3.1.4.11 The main form found in eukaryotes, especially mammals.
- Zinc dependent phospholipase C family of bacterial enzymes EC 3.1.4.3 that includes alpha toxins
- Phosphatidylinositol diacylglycerol-lyase EC 4.6.1.13 Another related bacterial enzyme.
- Glycosylphosphatidylinositol diacylglycerol-lyase EC 4.6.1.14 A trypanosomal enzyme.
References
- ^ a b c d e "Gq alpha subunit". Wikipedia. Archived from the original on 2009-03-03. http://en.wikipedia.org/w/index.php?title=Gq_alpha_subunit&oldid=249458259. Retrieved on 2009-03-03.
- ^ a b Walter F., PhD. Boron (2003). Medical Physiology: A Cellular And Molecular Approaoch. Elsevier/Saunders. pp. 1300. ISBN 1-4160-2328-3. Page 104
- ^ GeneGlobe -> GHRH Signaling Retrieved on May 31, 2009
- ^ a b c Alberts B, Lewis J, Raff M, Roberts K, Walter P (2002). Molecular biology of the cell (4th Edition ed.). New York: Garland Science. ISBN 0-8153-3218-1.
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